A spectrophotometer, also known as a spectrometer, is a scientific instrument that decomposes complex light into spectral lines. The measurement range generally includes a visible light region having a wavelength range of 380 to 780 nm and an ultraviolet light region having a wavelength range of 200 to 380 nm. Different light sources have their own specific emission spectra, so different illuminants can be used as the light source of the instrument. The emission spectrum of the tungsten lamp: the spectrum light of the wavelength of 380-780 nm emitted by the tungsten lamp source is refracted by the prism, and a continuous chromatogram composed of red, orange, yellow, green, blue, krypton and purple is obtained; the chromatogram can be used as visible light. The source of the spectrophotometer.
Simple principle of spectrophotometerThe spectrophotometer uses a light source that can generate multiple wavelengths, and passes through a series of spectroscopic devices to generate a light source of a specific wavelength. After the light source passes through the sample to be tested, part of the light source is absorbed, and the absorbance of the sample is calculated, thereby converting into a sample. concentration. The absorbance of the sample is proportional to the concentration of the sample.
The basic working principle of the spectrophotometerThe basic working principle of a spectrophotometer is based on the selectivity of matter for the absorption of light (the wavelength of light). Different substances have their own absorption bands, so when the spectrum after light dispersion passes through a solution, Light of certain wavelengths is absorbed by the solution. At a certain wavelength, the concentration of the substance in the solution has a proportional relationship with the degree of attenuation of the light energy, that is, according to Beer's law.
T = I/Io lg(Io/I)=εcb
In the formula, T is the transmittance, Io is the incident light intensity, I is the transmitted light intensity, A is the extinction value (absorbance), ε is the absorption coefficient, b is the optical path length of the solution, and c is the concentration of the solution. It can be seen from the above formula that when the incident light, the absorption coefficient, and the thickness of the solution are constant, the light transmittance varies depending on the concentration of the solution.
Spectrophotometers have become routine instruments in modern molecular biology laboratories. Often used for nucleic acid, protein quantification and quantification of bacterial growth concentrations.
Unique dual-optical, dual-beam optical system with higher resolution, lower stray light, better stability and reliability, and more accurate analysis;
Adopting 320*240 dot matrix highlighting 6†LCD display, the display is clear and the information is complete;
The unique long-path optical path design makes the instrument resolution higher, especially suitable for the powerful data processing function of micro-test, so that the test results can be fully applied, and the user's editing is simpler and faster;
Suspension optical system design, the overall optical path is independently fixed on the 16mm thick aluminum deformation-free base, the deformation of the bottom plate and the external vibration have no effect on the optical system, thus greatly improving the stability and reliability of the instrument. ;
Adopt synchronous sinusoidal mechanism, high wavelength accuracy and good repeatability;
Using an ARM system;
The 0.1/0.2/0.5/1.0/2.0/4.0 six-speed spectral bandwidth is automatically selected to meet the measurement needs of different users;
24-bit high-speed, high-precision A/D conversion, higher instrument accuracy and faster response;
The main components are imported, which makes the instrument less stray light, stable and reliable.
More powerful, the host can independently perform photometric measurement, quantitative measurement, spectral scanning, dynamics, DNA/protein testing, multi-wavelength testing and data printing;
Taking full account of the usage habits of different users, this series of instruments are equipped with the spectrum analysis software of Yuanfang Company. When online operation, in addition to all the test functions of the host, it can realize more powerful data processing functions and make data storage. Reach unlimited.
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